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These solutions were phosphate (100 mM)-EDTA (1 mM) buffers at pH 5.0, 7.4 or 8.0, or 42.5, 50, or 100 mM sodium carbonate solutions, resulting in pH values of 2.5, 6.2, 6.8, 8.4, 9.0, or 10 in the final samples, respectively
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