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Protons as versatile reporters in solid-state NMR spectroscopy

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His-tagged protein purification:- Recombinant proteins which have Histidine tags can be purified using immobilised metal-ion Chromatography (IMAC),and these tags can be placed at either the N-or C-terminus of the protein.Optimal binding and, therefore, purification efficiency is achieved when the His-tag is freely accessible to metal ion suppor