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On the next day, after the membrane was washed with TBST for five times, HRP-conjugated secondary antibody (Thermo Scientific) was applied for 1.5 h at room temperature

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In addition to Nanog and Oct4, Sox2 is an essential component of transcriptional networks controlling pluripotency of ESCs (Masui et al., 2007) and multipotency of hippocampal NPCs (Suh et al., 2007)